Richard Buller, Ph.D. D(ABMM)  Buller@kids.wustl.edu

Patient Oriented Research UnitLaboratory Medicine

phone: (314) 454-4525

Education

  • B.S., Michigan Technological University1972
  • M.S., Michigan Technological University1974
  • Ph.D., University of Montana1983

Training

  • Visiting Assistant Professor, University of Montana1982 - 1983
  • Visiting Instructor, Western Montana College1982 - 1983
  • Staff Fellow, NIH, NIAID Rocky Mountain Laboratories1984 - 1989
  • Postdoctoral Fellow, Washington University School of Medicine1989 - 1991

Licensure and Board Certification

  • American Academy of Microbiology - Registered Microbiologist1981
  • American Academy of Microbiology - Specialist, Public Health and Medical Laboratory Microbiology1984
  • American Board of Medical Microbiology (ABMM) - Diplomat: Clinical and Public Health Virology1993

Recent Publications view all (51)


Publication Co-Authors

  1. Crotty MP, Meyers S, Hampton N, Bledsoe S, Ritchie DJ, Buller RS, Storch GA, Micek ST, Kollef MH. Epidemiology, Co-Infections, and Outcomes of Viral Pneumonia in Adults: An Observational Cohort Study. Medicine (Baltimore). 2015;94(50):e2332. doi:10.1097/MD.0000000000002332  PMID:26683973  
  2. Crotty MP, Meyers S, Hampton N, Bledsoe S, Ritchie DJ, Buller RS, Storch GA, Kollef MH, Micek ST. Impact of antibacterials on subsequent resistance and clinical outcomes in adult patients with viral pneumonia: an opportunity for stewardship. Crit Care. 2015;19:404. doi:10.1186/s13054-015-1120-5  PMCID:PMC4650137  PMID:26577540  
  3. Beigelman A, Bacharier LB, Baty J, Buller R, Mason S, Schechtman KB, Sajol G, Isaacson-Schmid M, Castro M, Storch GA. Does azithromycin modify viral load during severe respiratory syncytial virus bronchiolitis? J Allergy Clin Immunol. 2015. doi:10.1016/j.jaci.2015.06.011  PMID:26215052  
  4. Wylie TN, Wylie KM, Buller RS, Cannella M, Storch GA. Development and Evaluation of an Enterovirus D68 Real-Time Reverse Transcriptase PCR Assay. J Clin Microbiol. 2015. doi:10.1128/JCM.00923-15  PMID:26063859  
  5. Wylie KM, Wylie TN, Orvedahl A, Buller RS, Herter BN, Magrini V, Wilson RK, Storch GA. Genome sequence of enterovirus D68 from St. Louis, Missouri, USA. Emerg Infect Dis. 2015;21(1):184-6. doi:10.3201/eid2101.141605  PMCID:PMC4285240  PMID:25532062  
  6. Rychert J, Danziger-Isakov L, Yen-Lieberman B, Storch G, Buller R, Sweet SC, Mehta AK, Cheeseman JA, Heeger P, Rosenberg ES, Fishman JA. Multicenter comparison of laboratory performance in cytomegalovirus and Epstein-Barr virus viral load testing using international standards. Clin Transplant. 2014;28(12):1416-23. doi:10.1111/ctr.12473  PMCID:PMC4481866  PMID:25303316  
  7. Buller RS. Molecular detection of respiratory viruses. Clin Lab Med. 2013;33(3):439-60. doi:10.1016/j.cll.2013.03.007  PMID:23931834  
  8. Colvin JM, Muenzer JT, Jaffe DM, Smason A, Deych E, Shannon WD, Arens MQ, Buller RS, Lee WM, Weinstock EJ, Weinstock GM, Storch GA. Detection of viruses in young children with fever without an apparent source. Pediatrics. 2012;130(6):e1455-62. doi:10.1542/peds.2012-1391  PMCID:PMC3507256  PMID:23129086  
  9. Yamada M, Buller R, Bledsoe S, Storch GA. Rising rates of macrolide-resistant Mycoplasma pneumoniae in the central United States. Pediatr Infect Dis J. 2012;31(4):409-0. doi:10.1097/INF.0b013e318247f3e0  PMID:22209916  
  10. Arens MQ, Buller RS, Rankin A, Mason S, Whetsell A, Agapov E, Lee WM, Storch GA. Comparison of the Eragen Multi-Code Respiratory Virus Panel with conventional viral testing and real-time multiplex PCR assays for detection of respiratory viruses. J Clin Microbiol. 2010;48(7):2387-95. doi:10.1128/JCM.00220-10  PMCID:PMC2897522  PMID:20484608  
  11. Hormozdi DJ, Arens MQ, Le BM, Buller RS, Agapov E, Storch GA. KI polyomavirus detected in respiratory tract specimens from patients in St. Louis, Missouri. Pediatr Infect Dis J. 2010;29(4):329-33. doi:10.1097/INF.0b013e3181c1795c  PMID:19949359  
  12. Nolte FS, Marshall DJ, Rasberry C, Schievelbein S, Banks GG, Storch GA, Arens MQ, Buller RS, Prudent JR. MultiCode-PLx system for multiplexed detection of seventeen respiratory viruses. J Clin Microbiol. 2007;45(9):2779-86. doi:10.1128/JCM.00669-07  PMCID:PMC2045250  PMID:17596361  
  13. Le B-M, Demertzis LM, Wu G, Tibbets RJ, Buller R, Arens MQ, Gaynor AM, Storch GA, Wang D. Clinical and epidemiologic characterization of WU polyomavirus infection, St. Louis, Missouri. Emerg Infect, Dis. 2007;13:1936-1938. 
  14. Elizur A, Orscheln RC, Ferkol TW, Atkinson JJ, Dunne WM Jr, Buller RS, Armstrong JR, Mardis ER, Storch GA, Cannon CL. Panton-Valentine Leukocidin-positive methicillin-resistant Staphylococcus aureus lung infection in patients with cystic fibrosis. Chest. 2007;131(6):1718-25. doi:10.1378/chest.06-2756  PMID:17400676  
  15. Harrington SM, Buller RS, Storch GA, Li L, Fischer SH, Murray PR, Gea-Banacloche JC. The effect of quantification standards used in real-time CMV PCR assays on guidelines for initiation of therapy in allogeneic stem cell transplant patients. Bone Marrow Transplant. 2007;39(4):237-8. doi:10.1038/sj.bmt.1705558  PMID:17211434  
  16. Liao RS, Storch GA, Buller RS, Orscheln RC, Mardis ER, Armstrong JR, Dunne WM Jr. Blinded comparison of repetitive-sequence PCR and multilocus sequence typing for genotyping methicillin-resistant Staphylococcus aureus isolates from a children's hospital in St. Louis, Missouri. J Clin Microbiol. 2006;44(6):2254-7. doi:10.1128/JCM.00690-06  PMCID:PMC1489435  PMID:16757633  
  17. Blaeser F, Kelly M, Siegrist K, Storch GA, Buller RS, Whitlock J, Truong N, Chatila TA. Critical function of the CD40 pathway in parvovirus B19 infection revealed by a hypomorphic CD40 ligand mutation. Clin Immunol. 2005;117(3):231-7. doi:10.1016/j.clim.2005.08.005  PMID:16169277  
  18. Brenner W, Storch G, Buller R, Vij R, Devine S, DiPersio J. Correspondence: West Nile virus encephalopathy in an allogeneic stem cell transplant recipient: Use of quantitative PCR for diagnosis and assessment of viral clearance. Bone Marrow Transplant. 2005;36:369-370. 
  19. Mulford WS, Buller RS, Arens MQ, Storch GA. Correlation of cerebrospinal fluid (CSF) cell counts and elevated CSF protein levels with enterovirus reverse transcription-PCR results in pediatric and adult patients. J Clin Microbiol. 2004;42(9):4199-203. doi:10.1128/JCM.42.9.4199-4203.2004  PMCID:PMC516328  PMID:15365011  
  20. Buller RS, Storch G. Evaluation of a real-time PCR assay using the LightCycler system for detection of parvovirus B19 DNA. J Clin Microbiol. 2004;42(7):3326-8. doi:10.1128/JCM.42.7.3326-3328.2004  PMCID:PMC446240  PMID:15243107  
  21. Arens MQ, Liddell AM, Buening G, Gaudreault-Keener M, Sumner JW, Comer JA, Buller RS, Storch GA. Detection of Ehrlichia spp. in the blood of wild white-tailed deer in Missouri by PCR assay and serologic analysis. J Clin Microbiol. 2003;41(3):1263-5. PMCID:PMC150261  PMID:12624063  
  22. Liddell AM, Sumner JW, Paddock CD, Rikihisa Y, Unver A, Buller RS, Storch GA. Reinfection with Ehrlichia chaffeensis in a liver transplant recipient. Clin Infect Dis. 2002;34(12):1644-7. doi:10.1086/340523  PMID:12032902  
  23. Paddock CD, Folk SM, Shore GM, Machado LJ, Huycke MM, Slater LN, Liddell AM, Buller RS, Storch GA, Monson TP, Rimland D, Sumner JW, Singleton J, Bloch KC, Tang YW, Standaert SM, Childs JE. Infections with Ehrlichia chaffeensis and Ehrlichia ewingii in persons coinfected with human immunodeficiency virus. Clin Infect Dis. 2001;33(9):1586-94. doi:10.1086/323981  PMID:11568857  
  24. Gusa AA, Buller RS, Storch GA, Huycke MM, Machado LJ, Slater LN, Stockham SL, Massung RF. Identification of a p28 gene in Ehrlichia ewingii: evaluation of gene for use as a target for a species-specific PCR diagnostic assay. J Clin Microbiol. 2001;39(11):3871-6. doi:10.1128/JCM.39.11.3871-3876.2001  PMCID:PMC88457  PMID:11682500  
  25. Young PP, Buller RS, Storch GA. Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens. J Clin Microbiol. 2000;38(11):4260-1. PMCID:PMC87577  PMID:11060104  
  26. Buller RS. Ehrlichia ewingii: an emerging agent of human ehrlichiosis. LabMedica International. 2000;March/April:19-21. 
  27. Wong M, Schlaggar BL, Buller RS, Storch GA, Landt M. Cerebrospinal fluid protein concentration in pediatric patients: defining clinically relevant reference values. Arch Pediatr Adolesc Med. 2000;154(8):827-31. PMID:10922281  
  28. Sumner JW, Storch GA, Buller RS, Liddell AM, Stockham SL, Rikihisa Y, Messenger S, Paddock CD. PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris. J Clin Microbiol. 2000;38(7):2746-9. PMCID:PMC87017  PMID:10878077  
  29. Unver A, Rikihisa Y, Ohashi N, Cullman LC, Buller R, Storch GA. Western and dot blotting analyses of Ehrlichia chaffeensis indirect fluorescent-antibody assay-positive and -negative human sera by using native and recombinant E. chaffeensis and E. canis antigens. J Clin Microbiol. 1999;37:3888-3895. 
  30. Buller RS, Arens M, Hmiel SP, Paddock CD, Sumner JW, Rikhisa Y, Unver A, Gaudreault-Keener M, Manian FA, Liddell AM, Schmulewitz N, Storch GA. Ehrlichia ewingii, a newly recognized agent of human ehrlichiosis. N Engl J Med. 1999;341(3):148-55. doi:10.1056/NEJM199907153410303  PMID:10403852  
  31. Roberts TC, Brennan DC, Buller RS, Gaudreault-Keener M, Schnitzler MA, Sternhell KE, Garlock KA, Singer GG, Storch GA. Quantitative polymerase chain reaction to predict occurrence of symptomatic cytomegalovirus infection and assess response to ganciclovir therapy in renal transplant recipients. J Infect Dis. 1998;178(3):626-35. PMID:9728529  
  32. Brennan DC, Garlock KA, Singer GG, Schnitzler MA, Lippmann BJ, Buller RS, Gaudreault-Keener M, Lowell JA, Shenoy S, Howard TK, Storch GA. Prophylactic oral ganciclovir compared with deferred therapy for control of cytomegalovirus in renal transplant recipients. Transplantation. 1997;64(12):1843-6. PMID:9422429  
  33. Roberts TC, Buller RS, Gaudreault-Keener M, Sternhell KE, Garlock K, Singer GG, Brennan DC, Storch GA. Effects of storage temperature and time on qualitative and quantitative detection of cytomegalovirus in blood specimens by shell vial culture and PCR. J Clin Microbiol. 1997;35(9):2224-8. PMCID:PMC229944  PMID:9276392  
  34. Brennan DC, Garlock KA, Lippmann BJ, Buller RS, Gaudreault-Keener M, Lowell JA, Miller SB, Shenoy S, Howard TK, Storch GA. Polymerase chain reaction-triggered preemptive or deferred therapy to control cytomegalovirus-associated morbidity and costs in renal transplant patients. Transplant Proc. 1997;29(1-2):809-11. PMID:9123536  
  35. Brennan DC, Garlock KA, Lippmann BA, Buller RS, Gaudreault-Keener M, Lowell JA, Miller SB, Shenoy S, Howard TK, Storch GA. Control of cytomegalovirus-associated morbidity in renal transplant patients using intensive monitoring and either preemptive or deferred therapy. J Am Soc Nephrol. 1997;8(1):118-25. PMID:9013456  
  36. Buller RS, Gaudreault-Keener M, Rossiter-Fornoff J, Storch GA. Direct quantitative comparison of shell vial and conventional culture for detection of CMV viremia. Clin Diagn Virol. 1995;3(4):317-22. PMID:15566812  
  37. Bailey TC, Buller RS, Ettinger NA, Trulock EP, Gaudreault-Keener M, Langlois TM, Fornoff JE, Cooper JD, Storch GA. Quantitative analysis of cytomegalovirus viremia in lung transplant recipients. J Infect Dis. 1995;171(4):1006-10. PMID:7706777  
  38. Schlesinger Y, Tebas P, Gaudreault-Keener M, Buller RS, Storch GA. Herpes simplex virus type 2 meningitis in the absence of genital lesions: improved recognition with use of the polymerase chain reaction. Clin Infect Dis. 1995;20(4):842-8. PMID:7795083  
  39. Schlesinger Y, Buller RS, Brunstrom JE, Moran CJ, Storch GA. Expanded spectrum of herpes simplex encephalitis in childhood. J Pediatr. 1995;126(2):234-41. PMID:7844669  
  40. Lee SF, Buller R, Chansue E, Hanika WC Brunt EM, Aquino T, Storch GA, Pepose JS. Vaccinia keratouveitis presenting as a masquerade syndrome Am J Ophthal. 1994;117:480-487. 
  41. Storch GA, Buller RS, Bailey TC, Ettinger NA, Langlois T, Gaudreault-Keener M, Welby PL. Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients. J Clin Microbiol. 1994;32(4):997-1003. PMCID:PMC267169  PMID:8027357  
  42. Clifford DB, Buller RS, Mohammed S, Robison L, Storch GA. Use of polymerase chain reaction to demonstrate cytomegalovirus DNA in CSF of patients with human immunodeficiency virus infection. Neurology. 1993;43(1):75-9. PMID:8380909  
  43. Buller RS, Bailey TC, Ettinger NA, Keener M, Langlois T, Miller JP, Storch GA. Use of a modified shell vial technique to quantitate cytomegalovirus viremia in a population of solid-organ transplant recipients. J Clin Microbiol. 1992;30(10):2620-4. PMCID:PMC270488  PMID:1328280  
  44. Pincus SH, Wehrly K, Tschachler E, Hayes SF, Buller RS, Reitz M. Variants selected by treatment of human immunodeficiency virus-infected cells with an immunotoxin. J Exp Med. 1990;172(3):745-57. PMCID:PMC2188557  PMID:1696955  
  45. Chesebro B, Buller R, Portis J, Wehrly K. Failure of human immunodeficiency virus entry and infection in CD4-positive human brain and skin cells. J Virol. 1990;64:214-221. 
  46. Caughey B, Neary K, Buller R, Ernst D, Perry LL, Chesebro B, Race RE. Normal and scrapie-associated forms of prion protein differ in their sensitivities to phospholipase and proteases in intact neuroblastoma cells. J Virol. 1990;64:1093-1101. 
  47. Buller RS, Wehrly K, Portis JL, Chesebro B. Host genes conferring resistance to a central nervous system disease induced by a polytropic recombinant Friend murine retrovirus. J Virol. 1990;64(2):493-8. PMCID:PMC249136  PMID:2153219  
  48. Buller RS, Van Zant G, Eldridge PW, Portis JL. A population of murine hematopoietic progenitors expresses an endogenous retroviral gp70 linked to the Rmcf gene and associated with resistance to erythroleukemia. J Exp Med. 1989;169(3):865-80. PMCID:PMC2189291  PMID:2926325  
  49. Buller RS, Sitbon M, Portis JL. The endogenous mink cell focus-forming (MCF) gp70 linked to the Rmcf gene restricts MCF virus replication in vivo and provides partial resistance to erythroleukemia induced by Friend murine leukemia virus. J Exp Med. 1988;167(5):1535-46. PMCID:PMC2188947  PMID:2835418  
  50. Remington KM, Buller RS, Kelly JR. Effect of the Today contraceptive sponge on growth and toxic shock syndrome toxin-1 production by Staphylococcus aureus. Obstet Gynecol. 1987;69(4):563-9. PMID:3822297  
  51. Buller RS, Ahmed A, Portis JL. Identification of two forms of an endogenous murine retroviral env gene linked to the Rmcf locus. J Virol. 1987;61(1):29-34. PMCID:PMC255194  PMID:3023705  
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