Department of Pediatrics

Clinical Interests

Dr.Horani is a member of the Division of Allergy, Immunology and Pulmonary Medicine. He was born in Israel and received his medical degree from the Hebrew University of Jerusalem. Dr. Horani completed a residency in Pediatrics at the Hadassah-Hebrew University Medical Center in Jerusalem between 2004 and 2009. He then pursued a fellowship in Pediatric Pulmonology at Washington University School of Medicine in St. Louis before joining the faculty of Washington University in 2014. Dr. Horani runs the PCD and Rare Airway Disease Center at Washington University Department of Pediatrics, where he evaluates and treats patients with complex rare lung disease and Primary Ciliary Dyskinesia, a genetic condition that results in impaired mucociliary clearance, causing progressive involvement of the respiratory tract characterized by recurrent infections of the lungs, middle ear, and paranasal sinuses.Dr Horani's clinical interests include early childhood lung disease, cystic fibrosis, Primary Ciliary Dyskinesia, and asthma. Dr. Horani is the recipient of multiple scientific awards related to his work in pediatric research. Working alongside members of a multidisciplinary team at Washington University in St. Louis, Dr. Horani's work in the laboratory focuses on airway cell differentiation and pathogenesis in disease, the importance of genes associated with the Primary Ciliary Dyskinesia, and the function of proteins important in the assembly of motile cilia, a structure known to be defective in PCD.

Education

• BSc, with distinction, The Hebrew University of Jerusalem, 1999
• MD, The Hebrew University of Jerusalem, 2004

Training

• Pediatric Residency, Hadassah-Hebrew University Medical Center, 2004 - 2009
• Fellowship in Pulmonary Medicine, St. Louis Children's Hospital, 2010 - 2013
• Advanced fellowship in Pediatric Pulmonary Medicine, St. Louis Children's Hospital, 2013 - 2014

Licensure and Board Certification

• 2004Israeli Medical License
• 2009Israeli Board of Pediatrics
• 2014MO, Missouri Medical License
• 2015Israeli Board of Pediatric Pulmonary Medicine

Honors and Awards

• Excellent clinical Teacher, Department of Pediatrics, Hadassah-Hebrew University Medical Center., 2005
• Excellent clinical Teacher, Department of Pediatrics, Hadassah-Hebrew University Medical Center., 2006
• Dr. BOYKES Honorary award for excellent Pediatric Research, Israeli Association of Clinical Pediatrics, 2007
• Young Clinician Award, Hadassah-Hebrew University Medical Center, 2008
• Dr. DIMSON Honorary Faculty award for excellent Pediatric Research, The Hebrew University-Hadassah Faculty of Medicine, 2008
• Best fellows abstract, pediatric research retreat, Washington University in St. Louis, 2012
• Martin K. and Jill F. Sneider Endowed Fellowship Award, 2013
• Annual ATS Assembly on Pediatrics Scientific Award, 2013
• The Japanese Respiratory Society International Session Award, 2014
• Gordon conference Abstract Award, 2015

Recent Publications view all (48)

Publication Co-Authors

Joshua Blatter, M.D., M.P.H.

Zhuoming Liu

Jessica Pittman, M.D., M.P.H.

Cadence Kuklinski, D.O.

David W. Piston, PhD

Maleewan Kitcharoensakkul, MD, MSCI

Steven Brennan, MBBS

Washington University has a long tradition of collaboration amongst colleagues.The faculty above have co-authored publications with Amjad Horani, M.D.

1. Konjikusic MJ, Lee C, Yue Y, Shrestha BD, Nguimtsop AM, Horani A, Brody S, Prakash VN, Gray RS, Verhey KJ, Wallingford JB. Kif9 is an active kinesin motor required for ciliary beating and proximodistal patterning of motile axonemes. J Cell Sci. 2023;136(5). PMID:35531639 
2. Puray-Chavez M, Lee N, Tenneti K, Wang Y, Vuong HR, Liu Y, Horani A, Huang T, Gunsten SP, Case JB, Yang W, Diamond MS, Brody SL, Dougherty J, Kutluay SB. The Translational Landscape of SARS-CoV-2-infected Cells Reveals Suppression of Innate Immune Genes. mBio. 2022;13(3):e0081522. PMCID:PMC9239271  PMID:35604092 
3. Caliskan AB, Horani A, Manion M, Brody SL. Variations in infection control practices suggest a need for guidelines in primary ciliary dyskinesia patient care. Pediatr Pulmonol. 2022;57(4):1072-1075. PMID:35043594 
4. O'Connor MG, Horani A, Shapiro AJ. Progress in Diagnosing Primary Ciliary Dyskinesia: The North American Perspective. Diagnostics (Basel). 2021;11(7). PMCID:PMC8304305  PMID:34359360 
5. Puray-Chavez M, LaPak KM, Schrank TP, Elliott JL, Bhatt DP, Agajanian MJ, Jasuja R, Lawson DQ, Davis K, Rothlauf PW, Liu Z, Jo H, Lee N, Tenneti K, Eschbach JE, Shema Mugisha C, Cousins EM, Cloer EW, Vuong HR, VanBlargan LA, Bailey AL, Gilchuk P, Crowe JE Jr, Diamond MS, Hayes DN, Whelan SPJ, Horani A, Brody SL, Goldfarb D, Major MB, Kutluay SB. Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell. Cell Rep. 2021;36(2):109364. PMCID:PMC8220945  PMID:34214467 
6. Puray-Chavez M, LaPak KM, Schrank TP, Elliott JL, Bhatt DP, Agajanian MJ, Jasuja R, Lawson DQ, Davis K, Rothlauf PW, Jo H, Lee N, Tenneti K, Eschbach JE, Mugisha CS, Vuong HR, Bailey AL, Hayes DN, Whelan SPJ, Horani A, Brody SL, Goldfarb D, Major MB, Kutluay SB. Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell. bioRxiv. 2021. PMCID:PMC7941617  PMID:33688646 
7. Brennan SK, Molter D, Menezes M, Dunsky K, Leonard D, Lieu J, Hirose K, Hazan G, Horani A, Ferkol T, Brody SL. Implementation of a screening tool for primary ciliary dyskinesia (PCD) in a pediatric otolaryngology clinic. Int J Pediatr Otorhinolaryngol. 2020;142:110586. PMID:33388601 
8. Lee C, Cox RM, Papoulas O, Horani A, Drew K, Devitt CC, Brody SL, Marcotte EM, Wallingford JB. Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins. Elife. 2020;9. PMCID:PMC7785291  PMID:33263282 
9. Horani A, Ferkol TW. Understanding Primary Ciliary Dyskinesia and Other Ciliopathies. J Pediatr. 2020. PMID:33242470 
10. Puray-Chavez M, Tenneti K, Vuong HR, Lee N, Liu Y, Horani A, Huang T, Case JB, Yang W, Diamond MS, Brody SL, Dougherty J, Kutluay SB. The translational landscape of SARS-CoV-2 and infected cells. bioRxiv. 2020. PMCID:PMC7654850  PMID:33173862 
11. Hawkins FJ, Suzuki S, Beermann ML, Barillà C, Wang R, Villacorta-Martin C, Berical A, Jean JC, Le Suer J, Matte T, Simone-Roach C, Tang Y, Schlaeger TM, Crane AM, Matthias N, Huang SXL, Randell SH, Wu J, Spence JR, Carraro G, Stripp BR, Rab A, Sorsher EJ, Horani A, Brody SL, Davis BR, Kotton DN. Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2020. PMID:33098807 
12. Gileles-Hillel A, Mor-Shaked H, Shoseyov D, Reiter J, Tsabari R, Hevroni A, Cohen-Cymberknoh M, Amirav I, Brammli-Greenberg S, Horani A, Kerem E, Breuer O. Whole-exome sequencing accuracy in the diagnosis of primary ciliary dyskinesia. ERJ Open Res. 2020;6(4). PMCID:PMC7792814  PMID:33447612 
13. Bustamante-Marin XM, Horani A, Stoyanova M, Charng WL, Bottier M, Sears PR, Yin WN, Daniels LA, Bowen H, Conrad DF, Knowles MR, Ostrowski LE, Zariwala MA, Dutcher SK. Mutation of CFAP57, a protein required for the asymmetric targeting of a subset of inner dynein arms in Chlamydomonas, causes primary ciliary dyskinesia. PLoS Genet. 2020;16(8):e1008691. PMID:32764743 
14. Moore PE, Boyer D, Perkins R, Katz ES, Castro-Codesal ML, MacLean JE, Akil N, Esther CR Jr, Kaslow J, Lewis TC, Krone KA, Quizon A, Simpson R, Benscoter D, Spielberg DR, Melicoff E, Kuklinski CA, Blatter JA, Dy J, Rettig JS, Horani A, Gross J. American Thoracic Society 2019 Pediatric Core Curriculum. Pediatr Pulmonol. 2019. PMID:31456278 
15. Horani A, Brody SL. Frequenting Sequencing: How Genetics Teaches us Cilia Biology. Am J Respir Cell Mol Biol. 2019. PMID:30951371 
16. Leigh MW, Horani A, Kinghorn B, O'Connor MG, Zariwala MA, Knowles MR. Primary Ciliary Dyskinesia (PCD): A genetic disorder of motile cilia. Transl Sci Rare Dis. 2019;4(1-2):51-75. PMCID:PMC6768089  PMID:31572664 
17. Huizar RL, Lee C, Boulgakov AA, Horani A, Tu F, Marcotte EM, Brody SL, Wallingford JB. A liquid-like organelle at the root of motile ciliopathy. Elife. 2018;7. PMCID:PMC6349401  PMID:30561330 
18. Horani A, Ferkol TW. Advances in the Genetics of Primary Ciliary Dyskinesia: Clinical Implications. Chest. 2018. PMID:29800551 
19. Horani A, Ustione A, Huang T, Firth AL, Pan J, Gunsten SP, Haspel JA, Piston DW, Brody SL. Establishment of the early cilia preassembly protein complex during motile ciliogenesis. Proc Natl Acad Sci U S A. 2018;115(6):E1221-E1228. PMCID:PMC5819421  PMID:29358401 
20. Moore PE, Poston JT, Boyer D, Barsky E, Gaffin J, Boyne KB, Ross KR, Mann Dosier LB, Vece TJ, Casey AM, Welsh SK, Logan JW, Shepherd EG, Phinzy PA, Panitch HB, Papantonakis CM, Austin ED, Orandi AB, Kitcharoensakkul M, Abe MK, Horani A, Rettig JS, Pittman J. ATS Core Curriculum 2017: Part II. Pediatric Pulmonary Medicine. Ann Am Thorac Soc. 2017;14(Supplement_2):S165-S181. PMID:28857623 
21. Horani A, Ferkol TW. Primary ciliary dyskinesia and associated sensory ciliopathies. Expert Rev Respir Med. 2016;10(5):569-76. doi:10.1586/17476348.2016.1165612  PMCID:PMC4893162  PMID:26967669 
22. Horani A, Ferkol TW, Dutcher SK, Brody SL. Genetics and biology of primary ciliary dyskinesia. Paediatr Respir Rev. 2015. doi:10.1016/j.prrv.2015.09.001  PMID:26476603 
23. Horani A, Brody SL, Ferkol TW. Response to Snijders et al. Pediatr Res. 2014;76(3):322. PMID:24933009 
24. Horani A, Brody SL, Ferkol TW. Picking up speed: advances in the genetics of primary ciliary dyskinesia. Pediatr Res. 2014;75(1-2):158-64. doi:10.1038/pr.2013.200  PMCID:PMC3946436  PMID:24192704 
25. Ghadially H, Horani A, Glasner A, Elboim M, Gazit R, Shoseyov D, Mandelboim O. NKp46 regulates allergic responses. Eur J Immunol. 2013;43(11):3006-16. doi:10.1002/eji.201343388  PMCID:PMC3867659  PMID:23878025 
26. Horani A, Nath A, Wasserman MG, Huang T, Brody SL. Rho-associated protein kinase inhibition enhances airway epithelial Basal-cell proliferation and lentivirus transduction. Am J Respir Cell Mol Biol. 2013;49(3):341-7. doi:10.1165/rcmb.2013-0046TE  PMCID:PMC3824057  PMID:23713995 
27. Horani A, Brody SL, Ferkol TW, Shoseyov D, Wasserman MG, Ta-shma A, Wilson KS, Bayly PV, Amirav I, Cohen-Cymberknoh M, Dutcher SK, Elpeleg O, Kerem E. CCDC65 mutation causes primary ciliary dyskinesia with normal ultrastructure and hyperkinetic cilia. PLoS One. 2013;8(8):e72299. doi:10.1371/journal.pone.0072299  PMCID:PMC3753302  PMID:23991085 
28. Horani A, Ferkol TW, Shoseyov D, Wasserman MG, Oren YS, Kerem B, Amirav I, Cohen-Cymberknoh M, Dutcher SK, Brody SL, Elpeleg O, Kerem E. LRRC6 mutation causes primary ciliary dyskinesia with dynein arm defects. PLoS One. 2013;8(3):e59436. doi:10.1371/journal.pone.0059436  PMCID:PMC3602302  PMID:23527195 
29. Horani A, Dickinson JD, Brody SL. Applications of mouse airway epithelial cell culture for asthma research. Methods Mol Biol. 2013;1032:91-107. doi:10.1007/978-1-62703-496-8_7  PMID:23943446 
30. Horani A, Druley TE, Zariwala MA, Patel AC, Levinson BT, Van Arendonk LG, Thornton KC, Giacalone JC, Albee AJ, Wilson KS, Turner EH, Nickerson DA, Shendure J, Bayly PV, Leigh MW, Knowles MR, Brody SL, Dutcher SK, Ferkol TW. Whole-exome capture and sequencing identifies HEATR2 mutation as a cause of primary ciliary dyskinesia. Am J Hum Genet. 2012;91(4):685-93. doi:10.1016/j.ajhg.2012.08.022  PMCID:PMC3484505  PMID:23040496 
31. Horani A, Shoseyov D, Ginsburg I, Mruwat R, Doron S, Amer J, Safadi R. Triphala (PADMA) extract alleviates bronchial hyperreactivity in a mouse model through liver and spleen immune modulation and increased anti-oxidative effects. Ther Adv Respir Dis. 2012;6(4):199-210. doi:10.1177/1753465812452194  PMID:22763675 
32. Jain R, Javidan-Nejad C, Alexander-Brett J, Horani A, Cabellon MC, Walter MJ, Brody SL. Sensory functions of motile cilia and implication for bronchiectasis. Front Biosci (Schol Ed). 2012;4:1088-98. PMCID:PMC3841983  PMID:22202111 
33. Horani A, Shoseyov D, Doron S, Mruwat R, Amer J, Kerem E, Safadi R. Immune modulation of ovalbumin-induced lung injury in mice using β-glucosylceramide and a potential role of the liver. Immunobiology. 2011;216(5):548-57. doi:10.1016/j.imbio.2010.10.002  PMID:21074892 
34. Ginsburg I, Koren E, Horani A, Mahamid M, Doron S, Muhanna N, Amer J, Safadi R. Amelioration of hepatic fibrosis via Padma Hepaten is associated with altered natural killer T lymphocytes. Clin Exp Immunol. 2009;157(1):155-64. doi:10.1111/j.1365-2249.2009.03936.x  PMCID:PMC2710603  PMID:19659781 
35. Orlev A, Horani A, Rapson Y, Cohen MJ, Blumenthal EZ. Clinical characteristics of eyes demonstrating atypical patterns in scanning laser polarimetry. Eye (Lond). 2008;22(11):1378-83. doi:10.1038/sj.eye.6702897  PMID:17627289 
36. Muhanna N, Doron S, Wald O, Horani A, Eid A, Pappo O, Friedman SL, Safadi R. Activation of hepatic stellate cells after phagocytosis of lymphocytes: A novel pathway of fibrogenesis. Hepatology. 2008;48(3):963-77. doi:10.1002/hep.22413  PMCID:PMC2880478  PMID:18726940 
37. Higazi AA, El-Haj M, Melhem A, Horani A, Pappo O, Alvarez CE, Muhanna N, Friedman SL, Safadi R. Immunomodulatory effects of plasminogen activators on hepatic fibrogenesis. Clin Exp Immunol. 2008;152(1):163-73. doi:10.1111/j.1365-2249.2008.03606.x  PMCID:PMC2384070  PMID:18279442 
38. Muhanna N, Horani A, Doron S, Safadi R. Lymphocyte-hepatic stellate cell proximity suggests a direct interaction. Clin Exp Immunol. 2007;148(2):338-47. doi:10.1111/j.1365-2249.2007.03353.x  PMCID:PMC1868876  PMID:17437422 
39. Horani A, Muhanna N, Pappo O, Melhem A, Alvarez CE, Doron S, Wehbi W, Dimitrios K, Friedman SL, Safadi R. Beneficial effect of glatiramer acetate (Copaxone) on immune modulation of experimental hepatic fibrosis. Am J Physiol Gastrointest Liver Physiol. 2007;292(2):G628-38. doi:10.1152/ajpgi.00137.2006  PMID:17038628 
40. Horani A, Frenkel S, Blumenthal EZ. Test-retest variability in visual field testing using frequency doubling technology. Eur J Ophthalmol. 2007;17(2):203-7. PMID:17415693 
41. Melhem A, Muhanna N, Bishara A, Alvarez CE, Ilan Y, Bishara T, Horani A, Nassar M, Friedman SL, Safadi R. Anti-fibrotic activity of NK cells in experimental liver injury through killing of activated HSC. J Hepatol. 2006;45(1):60-71. doi:10.1016/j.jhep.2005.12.025  PMID:16515819 
42. Blumenthal EZ, Horani A, Sasikumar R, Garudadri C, Udaykumar A, Thomas R. Correlating structure with function in end-stage glaucoma. Ophthalmic Surg Lasers Imaging. 2006;37(3):218-23. PMID:16749258 
43. Frenkel S, Katz S, Horani A, Kaiserman I, Asleh SA, Blumenthal EZ. Estimating intraocular pressure using a glass rod. Ann Ophthalmol (Skokie). 2006;38(3):195-9. PMID:17416953 
44. Horani A, Frenkel S, Blumenthal EZ. The effect of pupil dilation on scanning laser polarimetry with variable corneal compensation. Ophthalmic Surg Lasers Imaging. 2006;37(3):212-6. PMID:16749257 
45. Frenkel S, Slonim E, Horani A, Molcho M, Barzel I, Blumenthal EZ. Operator learning effect and interoperator reproducibility of the scanning laser polarimeter with variable corneal compensation. Ophthalmology. 2005;112(2):257-61. doi:10.1016/j.ophtha.2004.08.013  PMID:15691560 
46. Horani A, Ulitsky J, Kalish Y, Safadi R. Hodgkin's disease in pregnancy. Isr Med Assoc J. 2004;6(8):502-3. PMID:15326837 
47. Blumenthal EZ, Haddad A, Horani A, Anteby I. The reliability of frequency-doubling perimetry in young children. Ophthalmology. 2004;111(3):435-9. doi:10.1016/j.ophtha.2003.06.018  PMID:15019315 
48. Horani A, Frenkel S, Yahalom C, Farber MD, Ticho U, Blumenthal EZ. The learning effect in visual field testing of healthy subjects using frequency doubling technology. J Glaucoma. 2002;11(6):511-6. PMID:12483097