Dr. Kristine Wylie is an Assistant Professor in the Department of Pediatrics at Washington University School of Medicine and the McDonnell Genome Institute. Her main research interest is the human microbiome and its relationship to health and disease. During Dr. Wylie’s postdoctoral work, she developed methods for analyzing high-throughput metagenomic shotgun sequence data sets to study the human virome, the viral component of the microbiome. Dr. Wylie analyzed the human virome (eukaryotic viruses) in the healthy subjects from the Human Microbiome Project, providing the first analysis of the commensal virome in a large cohort of generally healthy, asymptomatic people. With Dr. Gregory Storch in Pediatrics, she studied the viromes in children in a study aimed at understanding the role of viruses in children with unexplained fevers. She and her colleagues determined that viruses are frequently found in samples from children with unexplained fever. Dr. Wylie and her colleagues recently developed a targeted sequence capture panel, ViroCap, designed to enrich nucleic acid from eukaryotic DNA and RNA viruses from 34 families that infect vertebrate hosts. This approach will greatly enhance the study of eukaryotic viruses and takes us closer to using high-throughput sequencing as a comprehensive viral diagnostic tool. Dr. Wylie is currently involved in many collaborative efforts that use high-throughput sequencing to study the microbiome and its association with disease.