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Cliff J. Luke, Ph.D.
cjluke@wustl.edu
Associate Professor of Pediatrics, Newborn Medicine
Newborn Medicine
phone: (314) 454-6148
Education
BSc,
Hons II(i)
,
University of Liverpool
,
1994
PhD,
University of Surrey
,
1998
Training
Postodoctoral Fellow, The Children's Hospital
,
1998
-
2001
Research fellow in Pediatrics, Harvard Medical School
,
1998
-
2001
Research Associate, The Children's Hospital
,
2001
-
2004
Honors and Awards
Aaron Janoff Recipient of the Young Investigator Award
,
2001
Recent Publications
view all (41)
Publication Co-Authors
Mary C. Dinauer, M.D., Ph.D.
Regina Clemens, M.D., Ph.D.
Jennifer A Wambach, M.D.
Anthony W. Orvedahl, M.D., Ph.D.
Lila Nolan
Stephen C. Pak, Ph.D.
Jocelyn Ou
David Perlmutter, M.D.
Gary A. Silverman, M.D., Ph.D.
Francis Sessions Cole, III, M.D.
Luke CJ, Markovina S, Good M, Wight IE, Thomas BJ, Linneman JM, Lanik WE, Koroleva O, Coffman MR, Miedel MT, Gong Q, Andress A, Campos Guerrero M, Wang S, Chen L, Beatty WL, Hausmann KN, White FV, Fitzpatrick JAJ, Orvedahl A, Pak SC, Silverman GA.
Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpins.
Commun Biol
.
2022;5
(1):
47.
PMCID:
PMC8755814
PMID:
35022507
Wang S, Luke CJ, Pak SC, Shi V, Chen L, Moore J, Andress AP, Jayachandran K, Zhang J, Huang Y, Platik M, Apicelli AA, Schwarz JK, Grigsby PW, Silverman GA, Markovina S.
SERPINB3 (SCCA1) inhibits cathepsin L and lysoptosis, protecting cervical cancer cells from chemoradiation.
Commun Biol
.
2022;5
(1):
46.
PMCID:
PMC8755728
PMID:
35022555
Mihi B, Gong Q, Nolan LS, Gale SE, Goree M, Hu E, Lanik WE, Rimer JM, Liu V, Parks OB, Lewis AN, Agrawal P, Laury ML, Kumar P, Huang E, Bidani SS, Luke CJ, Kolls JK, Good M.
Interleukin-22 signaling attenuates necrotizing enterocolitis by promoting epithelial cell regeneration.
Cell Rep Med
.
2021;2
(6):
100320.
PMCID:
PMC8233697
PMID:
34195684
Hu JY, Yang P, Wegner DJ, Heins HB, Luke CJ, Li F, White FV, Silverman GA, Sessions Cole F, Wambach JA.
Functional characterization of four ATP-binding cassette transporter A3 gene (ABCA3) variants.
Hum Mutat
.
2020;41
(7):
1298-1307.
PMCID:
PMC7292786
PMID:
32196812
Song Z, Huang G, Chiquetto Paracatu L, Grimes D, Gu J, Luke CJ, Clemens RA, Dinauer MC.
NADPH oxidase controls pulmonary neutrophil infiltration in the response to fungal cell walls by limiting LTB4.
Blood
.
2020;135
(12):
891-903.
PMCID:
PMC7082617
PMID:
31951647
Seiler KM, Bajinting A, Alvarado DM, Traore MA, Binkley MM, Goo WH, Lanik WE, Ou J, Ismail U, Iticovici M, King CR, VanDussen KL, Swietlicki EA, Gazit V, Guo J, Luke CJ, Stappenbeck T, Ciorba MA, George SC, Meacham JM, Rubin DC, Good M, Warner BW.
Patient-derived small intestinal myofibroblasts direct perfused, physiologically responsive capillary development in a microfluidic Gut-on-a-Chip Model.
Sci Rep
.
2020;10
(1):
3842.
PMCID:
PMC7051952
PMID:
32123209
Orvedahl A, McAllaster MR, Sansone A, Dunlap BF, Desai C, Wang YT, Balce DR, Luke CJ, Lee S, Orchard RC, Artyomov MN, Handley SA, Doench JG, Silverman GA, Virgin HW..
Autophagy genes in myeloid cells counteract IFNγ-induced TNF-mediated cell death and fatal TNF-induced shock.
PNAS
.
2019;116
(33):
16497-16506.
doi:
0.1073/pnas.1822157116
PMID:
31346084
Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, Silverman GA, Luke CJ.
CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans.
PLoS One
.
2019;14
(3):
e0214257.
doi:
10.1371/journal.pone.0214257
PMCID:
PMC6435234
PMID:
30913273
Lanik WE, Xu L, Luke CJ, Hu EZ, Agrawal P, Liu VS, Kumar R, Bolock AM, Ma C, Good M.
Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation.
J Vis Exp
.
2018;(132).
PMCID:
PMC5912412
PMID:
29553558
Markovina S, Wang S, Henke LE, Luke CJ, Pak SC, DeWees T, Pfeifer JD, Schwarz JK, Liu W, Chen S, Mutch D, Wang X, Powell MA, Siegel BA, Dehdashti F, Silverman GA, Grigsby PW.
Serum squamous cell carcinoma antigen as an early indicator of response during therapy of cervical cancer.
Br J Cancer
.
2017.
doi:
10.1038/bjc.2017.390
PMID:
29112685
Drummond CG, Bolock AM, Ma C, Luke CJ, Good M, Coyne CB.
Enteroviruses infect human enteroids and induce antiviral signaling in a cell lineage-specific manner.
Proc Natl Acad Sci U S A
.
2017;114
(7):
1672-1677.
PMCID:
PMC5320971
PMID:
28137842
Shi S, Luke CJ, Miedel MT, Silverman GA, Kleyman TR.
Activation of the Caenorhabditis elegans Degenerin Channel by Shear Stress Requires the MEC-10 Subunit.
J Biol Chem
.
2016;291
(27):
14012-22.
PMCID:
PMC4933161
PMID:
27189943
Niehaus JZ, Miedel MT, Good M, Wyatt AN, Pak SC, Silverman GA, Luke CJ.
SERPINB12 Is a Slow-Binding Inhibitor of Granzyme A and Hepsin.
Biochemistry
.
2015;54
(45):
6756-9.
PMCID:
PMC4900762
PMID:
26497600
Niehaus JZ, Good M, Jackson LE, Ozolek JA, Silverman GA, Luke CJ.
Human SERPINB12 Is an Abundant Intracellular Serpin Expressed in Most Surface and Glandular Epithelia.
J Histochem Cytochem
.
2015;63
(11):
854-65.
PMCID:
PMC4812677
PMID:
26220980
Cummings EE, O'Reilly LP, King DE, Silverman RM, Miedel MT, Luke CJ, Perlmutter DH, Silverman GA, Pak SC.
Deficient and Null Variants of SERPINA1 Are Proteotoxic in a Caenorhabditis elegans Model of α1-Antitrypsin Deficiency.
PLoS One
.
2015;10
(10):
e0141542.
PMCID:
PMC4626213
PMID:
26512890
Luke CJ, O'Reilly LP.
Microscopic Investigation of Protein Function in C. elegans Using Fluorescent Imaging.
Curr Protoc Cytom
.
2015;74:12.41.1-12.41.17.
PMID:
26423692
Silverman RM, Cummings EE, O'Reilly LP, Miedel MT, Silverman GA, Luke CJ, Perlmutter DH, Pak SC.
The aggregation-prone intracellular serpin SRP-2 fails to transit the ER in Caenorhabditis elegans.
Genetics
.
2015;200
(1):
207-19.
PMCID:
PMC4423363
PMID:
25786854
O'Reilly LP, Long OS, Cobanoglu MC, Benson JA, Luke CJ, Miedel MT, Hale P, Perlmutter DH, Bahar I, Silverman GA, Pak SC.
A genome-wide RNAi screen identifies potential drug targets in a C. elegans model of α1-antitrypsin deficiency.
Hum Mol Genet
.
2014;23
(19):
5123-32.
PMCID:
PMC4159156
PMID:
24838285
Long OS, Benson JA, Kwak JH, Luke CJ, Gosai SJ, O'Reilly LP, Wang Y, Li J, Vetica AC, Miedel MT, Stolz DB, Watkins SC, Züchner S, Perlmutter DH, Silverman GA, Pak SC.
A C. elegans model of human α1-antitrypsin deficiency links components of the RNAi pathway to misfolded protein turnover.
Hum Mol Genet
.
2014;23
(19):
5109-22.
PMCID:
PMC4159155
PMID:
24838286
Miedel MT, Zeng X, Yates NA, Silverman GA, Luke CJ.
Isolation of serpin-interacting proteins in C. elegans using protein affinity purification.
Methods
.
2014;68
(3):
536-41.
PMCID:
PMC4113314
PMID:
24798811
Luke CJ, Niehaus JZ, O'Reilly LP, Watkins SC.
Non-microfluidic methods for imaging live C. elegans.
Methods
.
2014;68
(3):
542-7.
PMCID:
PMC4812562
PMID:
24836996
O'Reilly LP, Luke CJ, Perlmutter DH, Silverman GA, Pak SC.
C. elegans in high-throughput drug discovery.
Adv Drug Deliv Rev
.
2014;69-70:247-53.
PMCID:
PMC4019719
PMID:
24333896
Chotoo CK, Silverman GA, Devor DC, Luke CJ.
A small conductance calcium-activated K+ channel in C. elegans, KCNL-2, plays a role in the regulation of the rate of egg-laying.
PLoS One
.
2013;8
(9):
e75869.
PMCID:
PMC3769271
PMID:
24040423
Miedel MT, Graf NJ, Stephen KE, Long OS, Pak SC, Perlmutter DH, Silverman GA, Luke CJ.
A pro-cathepsin L mutant is a luminal substrate for endoplasmic-reticulum-associated degradation in C. elegans.
PLoS One
.
2012;7
(7):
e40145.
PMCID:
PMC3388072
PMID:
22768338
Bhatia SR, Miedel MT, Chotoo CK, Graf NJ, Hood BL, Conrads TP, Silverman GA, Luke CJ.
Using C. elegans to identify the protease targets of serpins in vivo.
Methods Enzymol
.
2011;499:283-99.
PMCID:
PMC4374438
PMID:
21683259
Gosai SJ, Kwak JH, Luke CJ, Long OS, King DE, Kovatch KJ, Johnston PA, Shun TY, Lazo JS, Perlmutter DH, Silverman GA, Pak SC.
Automated high-content live animal drug screening using C. elegans expressing the aggregation prone serpin α1-antitrypsin Z.
PLoS One
.
2010;5
(11):
e15460.
PMCID:
PMC2980495
PMID:
21103396
Myerburg MM, McKenna EE, Luke CJ, Frizzell RA, Kleyman TR, Pilewski JM.
Prostasin expression is regulated by airway surface liquid volume and is increased in cystic fibrosis.
Am J Physiol Lung Cell Mol Physiol
.
2008;294
(5):
L932-41.
PMCID:
PMC2387194
PMID:
18310226
Luke CJ, Pak SC, Askew YS, Naviglia TL, Askew DJ, Nobar SM, Vetica AC, Long OS, Watkins SC, Stolz DB, Barstead RJ, Moulder GL, Brömme D, Silverman GA.
An intracellular serpin regulates necrosis by inhibiting the induction and sequelae of lysosomal injury.
Cell
.
2007;130
(6):
1108-19.
PMCID:
PMC2128786
PMID:
17889653
Askew DJ, Cataltepe S, Kumar V, Edwards C, Pace SM, Howarth RN, Pak SC, Askew YS, Brömme D, Luke CJ, Whisstock JC, Silverman GA.
SERPINB11 is a new noninhibitory intracellular serpin. Common single nucleotide polymorphisms in the scaffold impair conformational change.
J Biol Chem
.
2007;282
(34):
24948-60.
PMID:
17562709
Pak SC, Tsu C, Luke CJ, Askew YS, Silverman GA.
The Caenorhabditis elegans muscle specific serpin, SRP-3, neutralizes chymotrypsin-like serine peptidases.
Biochemistry
.
2006;45
(14):
4474-80.
PMCID:
PMC2654365
PMID:
16584183
Luke CJ, Pak SC, Askew DJ, Askew YS, Smith JE, Silverman GA.
Selective conservation of the RSL-encoding, proteinase inhibitory-type, clade L serpins in Caenorhabditis species.
Front Biosci
.
2006;11:581-94.
PMID:
16146754
Cole EB, Miller D, Rometo D, Greenberg RM, Brömme D, Cataltepe S, Pak SC, Mills DR, Silverman GA, Luke CJ.
Identification and activity of a lower eukaryotic serine proteinase inhibitor (serpin) from Cyanea capillata: analysis of a jellyfish serpin, jellypin.
Biochemistry
.
2004;43
(37):
11750-9.
PMID:
15362859
Askew DJ, Askew YS, Kato Y, Luke CJ, Pak SC, Brömme D, Silverman GA.
The amplified mouse squamous cell carcinoma antigen gene locus contains a serpin (Serpinb3b) that inhibits both papain-like cysteine and trypsin-like serine proteinases.
Genomics
.
2004;84
(1):
166-75.
PMID:
15203214
Pak SC, Kumar V, Tsu C, Luke CJ, Askew YS, Askew DJ, Mills DR, Brömme D, Silverman GA.
SRP-2 is a cross-class inhibitor that participates in postembryonic development of the nematode Caenorhabditis elegans: initial characterization of the clade L serpins.
J Biol Chem
.
2004;279
(15):
15448-59.
PMID:
14739286
Luke CJ.
Serpin production using rapid in vitro transcription/translation systems.
Methods
.
2004;32
(2):
191-8.
PMID:
14698632
Al-Khunaizi M, Luke CJ, Askew YS, Pak SC, Askew DJ, Cataltepe S, Miller D, Mills DR, Tsu C, Brömme D, Irving JA, Whisstock JC, Silverman GA.
The serpin SQN-5 is a dual mechanistic-class inhibitor of serine and cysteine proteinases.
Biochemistry
.
2002;41
(9):
3189-99.
PMID:
11863458
Askew YS, Pak SC, Luke CJ, Askew DJ, Cataltepe S, Mills DR, Kato H, Lehoczky J, Dewar K, Birren B, Silverman GA.
SERPINB12 is a novel member of the human ov-serpin family that is widely expressed and inhibits trypsin-like serine proteinases.
J Biol Chem
.
2001;276
(52):
49320-30.
PMID:
11604408
Phenix KV, Wark K, Luke CJ, Skinner MA, Smyth JA, Mawhinney KA, Todd D.
Recombinant Semliki Forest virus vector exhibits potential for avian virus vaccine development.
Vaccine
.
2001;19
(23-24):
3116-23.
PMID:
11312006
Uemura Y, Pak SC, Luke C, Cataltepe S, Tsu C, Schick C, Kamachi Y, Pomeroy SL, Perlmutter DH, Silverman GA.
Circulating serpin tumor markers SCCA1 and SCCA2 are not actively secreted but reside in the cytosol of squamous carcinoma cells.
Int J Cancer
.
2000;89
(4):
368-77.
PMID:
10956412
Luke C, Schick C, Tsu C, Whisstock JC, Irving JA, Brömme D, Juliano L, Shi GP, Chapman HA, Silverman GA.
Simple modifications of the serpin reactive site loop convert SCCA2 into a cysteine proteinase inhibitor: a critical role for the P3' proline in facilitating RSL cleavage.
Biochemistry
.
2000;39
(24):
7081-91.
PMID:
10852705
Cataltepe S, Schick C, Luke CJ, Pak SC, Goldfarb D, Chen P, Tanasiyevic MJ, Posner MR, Silverman GA.
Development of specific monoclonal antibodies and a sensitive discriminatory immunoassay for the circulating tumor markers SCCA1 and SCCA2.
Clin Chim Acta
.
2000;295
(1-2):
107-27.
PMID:
10767398
Last updated: 05/06/2022
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