Department of Pediatrics

Education

• BSc, Hons II(i), University of Liverpool, 1994
• PhD, University of Surrey, 1998

Training

• Postodoctoral Fellow, The Children's Hospital, 1998 - 2001
• Research fellow in Pediatrics, Harvard Medical School, 1998 - 2001
• Research Associate, The Children's Hospital, 2001 - 2004

Honors

• Aaron Janoff Recipient of the Young Investigator Award, 2001

Recent Publications view all (29)

Publication Co-Authors

Misty Good, M.D., M.S.

Stephen C. Pak, Ph.D.

David Perlmutter, M.D.

Gary A. Silverman, M.D., Ph.D.

Washington University has a long tradition of collaboration amongst colleagues.The faculty above have co-authored publications with Clifford J. Luke, Ph.D.

1. Shi S, Luke CJ, Miedel MT, Silverman GA, Kleyman TR. Activation of the Caenorhabditis elegans Degenerin Channel by Shear Stress Requires the MEC-10 Subunit. J Biol Chem. 2016;291(27):14012-22. PMCID:PMC4933161  PMID:27189943 
2. Niehaus JZ, Miedel MT, Good M, Wyatt AN, Pak SC, Silverman GA, Luke CJ. SERPINB12 Is a Slow-Binding Inhibitor of Granzyme A and Hepsin. Biochemistry. 2015;54(45):6756-9. PMCID:PMC4900762  PMID:26497600 
3. Niehaus JZ, Good M, Jackson LE, Ozolek JA, Silverman GA, Luke CJ. Human SERPINB12 Is an Abundant Intracellular Serpin Expressed in Most Surface and Glandular Epithelia. J Histochem Cytochem. 2015;63(11):854-65. PMCID:PMC4812677  PMID:26220980 
4. Cummings EE, O'Reilly LP, King DE, Silverman RM, Miedel MT, Luke CJ, Perlmutter DH, Silverman GA, Pak SC. Deficient and Null Variants of SERPINA1 Are Proteotoxic in a Caenorhabditis elegans Model of α1-Antitrypsin Deficiency. PLoS One. 2015;10(10):e0141542. PMCID:PMC4626213  PMID:26512890 
5. Silverman RM, Cummings EE, O'Reilly LP, Miedel MT, Silverman GA, Luke CJ, Perlmutter DH, Pak SC. The aggregation-prone intracellular serpin SRP-2 fails to transit the ER in Caenorhabditis elegans. Genetics. 2015;200(1):207-19. PMCID:PMC4423363  PMID:25786854 
6. O'Reilly LP, Long OS, Cobanoglu MC, Benson JA, Luke CJ, Miedel MT, Hale P, Perlmutter DH, Bahar I, Silverman GA, Pak SC. A genome-wide RNAi screen identifies potential drug targets in a C. elegans model of α1-antitrypsin deficiency. Hum Mol Genet. 2014;23(19):5123-32. PMCID:PMC4159156  PMID:24838285 
7. Long OS, Benson JA, Kwak JH, Luke CJ, Gosai SJ, O'Reilly LP, Wang Y, Li J, Vetica AC, Miedel MT, Stolz DB, Watkins SC, Züchner S, Perlmutter DH, Silverman GA, Pak SC. A C. elegans model of human α1-antitrypsin deficiency links components of the RNAi pathway to misfolded protein turnover. Hum Mol Genet. 2014;23(19):5109-22. PMCID:PMC4159155  PMID:24838286 
8. Miedel MT, Zeng X, Yates NA, Silverman GA, Luke CJ. Isolation of serpin-interacting proteins in C. elegans using protein affinity purification. Methods. 2014;68(3):536-41. PMCID:PMC4113314  PMID:24798811 
9. Luke CJ, Niehaus JZ, O'Reilly LP, Watkins SC. Non-microfluidic methods for imaging live C. elegans. Methods. 2014;68(3):542-7. PMCID:PMC4812562  PMID:24836996 
10. O'Reilly LP, Luke CJ, Perlmutter DH, Silverman GA, Pak SC. C. elegans in high-throughput drug discovery. Adv Drug Deliv Rev. 2014;69-70:247-53. PMCID:PMC4019719  PMID:24333896 
11. Chotoo CK, Silverman GA, Devor DC, Luke CJ. A small conductance calcium-activated K+ channel in C. elegans, KCNL-2, plays a role in the regulation of the rate of egg-laying. PLoS One. 2013;8(9):e75869. PMCID:PMC3769271  PMID:24040423 
12. Miedel MT, Graf NJ, Stephen KE, Long OS, Pak SC, Perlmutter DH, Silverman GA, Luke CJ. A pro-cathepsin L mutant is a luminal substrate for endoplasmic-reticulum-associated degradation in C. elegans. PLoS One. 2012;7(7):e40145. PMCID:PMC3388072  PMID:22768338 
13. Bhatia SR, Miedel MT, Chotoo CK, Graf NJ, Hood BL, Conrads TP, Silverman GA, Luke CJ. Using C. elegans to identify the protease targets of serpins in vivo. Methods Enzymol. 2011;499:283-99. PMCID:PMC4374438  PMID:21683259 
14. Gosai SJ, Kwak JH, Luke CJ, Long OS, King DE, Kovatch KJ, Johnston PA, Shun TY, Lazo JS, Perlmutter DH, Silverman GA, Pak SC. Automated high-content live animal drug screening using C. elegans expressing the aggregation prone serpin α1-antitrypsin Z. PLoS One. 2010;5(11):e15460. PMCID:PMC2980495  PMID:21103396 
15. Myerburg MM, McKenna EE, Luke CJ, Frizzell RA, Kleyman TR, Pilewski JM. Prostasin expression is regulated by airway surface liquid volume and is increased in cystic fibrosis. Am J Physiol Lung Cell Mol Physiol. 2008;294(5):L932-41. PMCID:PMC2387194  PMID:18310226 
16. Luke CJ, Pak SC, Askew YS, Naviglia TL, Askew DJ, Nobar SM, Vetica AC, Long OS, Watkins SC, Stolz DB, Barstead RJ, Moulder GL, Brömme D, Silverman GA. An intracellular serpin regulates necrosis by inhibiting the induction and sequelae of lysosomal injury. Cell. 2007;130(6):1108-19. PMCID:PMC2128786  PMID:17889653 
17. Askew DJ, Cataltepe S, Kumar V, Edwards C, Pace SM, Howarth RN, Pak SC, Askew YS, Brömme D, Luke CJ, Whisstock JC, Silverman GA. SERPINB11 is a new noninhibitory intracellular serpin. Common single nucleotide polymorphisms in the scaffold impair conformational change. J Biol Chem. 2007;282(34):24948-60. PMID:17562709 
18. Pak SC, Tsu C, Luke CJ, Askew YS, Silverman GA. The Caenorhabditis elegans muscle specific serpin, SRP-3, neutralizes chymotrypsin-like serine peptidases. Biochemistry. 2006;45(14):4474-80. PMCID:PMC2654365  PMID:16584183 
19. Luke CJ, Pak SC, Askew DJ, Askew YS, Smith JE, Silverman GA. Selective conservation of the RSL-encoding, proteinase inhibitory-type, clade L serpins in Caenorhabditis species. Front Biosci. 2006;11:581-94. PMID:16146754 
20. Cole EB, Miller D, Rometo D, Greenberg RM, Brömme D, Cataltepe S, Pak SC, Mills DR, Silverman GA, Luke CJ. Identification and activity of a lower eukaryotic serine proteinase inhibitor (serpin) from Cyanea capillata: analysis of a jellyfish serpin, jellypin. Biochemistry. 2004;43(37):11750-9. PMID:15362859 
21. Askew DJ, Askew YS, Kato Y, Luke CJ, Pak SC, Brömme D, Silverman GA. The amplified mouse squamous cell carcinoma antigen gene locus contains a serpin (Serpinb3b) that inhibits both papain-like cysteine and trypsin-like serine proteinases. Genomics. 2004;84(1):166-75. PMID:15203214 
22. Pak SC, Kumar V, Tsu C, Luke CJ, Askew YS, Askew DJ, Mills DR, Brömme D, Silverman GA. SRP-2 is a cross-class inhibitor that participates in postembryonic development of the nematode Caenorhabditis elegans: initial characterization of the clade L serpins. J Biol Chem. 2004;279(15):15448-59. PMID:14739286 
23. Luke CJ. Serpin production using rapid in vitro transcription/translation systems. Methods. 2004;32(2):191-8. PMID:14698632 
24. Al-Khunaizi M, Luke CJ, Askew YS, Pak SC, Askew DJ, Cataltepe S, Miller D, Mills DR, Tsu C, Brömme D, Irving JA, Whisstock JC, Silverman GA. The serpin SQN-5 is a dual mechanistic-class inhibitor of serine and cysteine proteinases. Biochemistry. 2002;41(9):3189-99. PMID:11863458 
25. Askew YS, Pak SC, Luke CJ, Askew DJ, Cataltepe S, Mills DR, Kato H, Lehoczky J, Dewar K, Birren B, Silverman GA. SERPINB12 is a novel member of the human ov-serpin family that is widely expressed and inhibits trypsin-like serine proteinases. J Biol Chem. 2001;276(52):49320-30. PMID:11604408 
26. Phenix KV, Wark K, Luke CJ, Skinner MA, Smyth JA, Mawhinney KA, Todd D. Recombinant Semliki Forest virus vector exhibits potential for avian virus vaccine development. Vaccine. 2001;19(23-24):3116-23. PMID:11312006 
27. Uemura Y, Pak SC, Luke C, Cataltepe S, Tsu C, Schick C, Kamachi Y, Pomeroy SL, Perlmutter DH, Silverman GA. Circulating serpin tumor markers SCCA1 and SCCA2 are not actively secreted but reside in the cytosol of squamous carcinoma cells. Int J Cancer. 2000;89(4):368-77. PMID:10956412 
28. Luke C, Schick C, Tsu C, Whisstock JC, Irving JA, Brömme D, Juliano L, Shi GP, Chapman HA, Silverman GA. Simple modifications of the serpin reactive site loop convert SCCA2 into a cysteine proteinase inhibitor: a critical role for the P3' proline in facilitating RSL cleavage. Biochemistry. 2000;39(24):7081-91. PMID:10852705 
29. Cataltepe S, Schick C, Luke CJ, Pak SC, Goldfarb D, Chen P, Tanasiyevic MJ, Posner MR, Silverman GA. Development of specific monoclonal antibodies and a sensitive discriminatory immunoassay for the circulating tumor markers SCCA1 and SCCA2. Clin Chim Acta. 2000;295(1-2):107-27. PMID:10767398