Department of Pediatrics

Clinical Interests

Dr. Rosen evaluates children with a variety of infectious diseases at St. Louis Children's Hospital. He has a particular interest in the treatment of resistant Gram-negative bacterial infections.

In the research lab, Dr. Rosen studies basic pathogenic mechanisms of Klebsiella pneumoniae. This organism often carries sets of genes that render it resistant to most, if not all, antibiotics available. Klebsiella, and other carbapenem-resistant Enterobacteriales (CRE), have recently been assigned a threat level of urgent by the CDC. Utilizing mouse models of pneumonia and urinary tract infection, Dr. Rosen dissects mechanisms by which Klebsiella pneumoniae infects the host. Specific interests includes differential regulation of virulence determinants, including type 1 pili and capsule, and adaptive immune responses to Klebsiella in the lung. The ultimate goal is to develop further understanding of Klebsiella pneumoniae pathogenesis, which may reveal alternative therapies to combat these resistant organisms.

Education

• BS, Highest Distinction and Highest Honors, University of Michigan, 2002
• MD, Washington University School of Medicine, 2010
• PhD, Washington University School of Medicine, 2010

Training

• Pediatric Internship and Residency, St. Louis Children's Hospital, 2010 - 2012
• Pediatric Infectious Disease Fellowship, Washington University School of Medicine, St. Louis Children's Hospital, 2012 - 2016

Licensure and Board Certification

• 2012 - PresMO, Physician and Surgeon License
• 2013 - PresGeneral Pediatrics, American Board of Pediatrics
• 2017 - PresPediatric Infectious Diseases, American Board of Pediatrics

Honors and Awards

• Farmington Rotary Foundation Daniel Waldman Memorial Scholarship, 1998
• State of Michigan House of Representatives Certificate of Recognition, 1998
• University of Michigan Regents-Alumni Scholarship, 1998
• William Beaumont Hospital Scholarship, 1998 - 2001
• University of Michigan James B. Angell Scholar, 1999 - 2002
• National Stark Scholarship for Chemistry and Biochemistry, 2001
• American Institute of Chemistry Merit Award for Biochemistry, 2002
• Washington University Medical School Teaching Assistant of the Year, 2007
• Washington University Teaching Citation, 2008
• Alexander Berg Prize for Microbiology Research, 2010
• St. Louis Children’s Hospital Chief Resident Award, 2012
• Pediatric Infectious Diseases Society Fellowship Award, 2013 - 2015
• Oliver Langenberg Physician-Scientist Training Program Post-Doctoral Trainee, Washington University School of Medicine, 2014 - 2017
• St. Louis Children’s Hospital Outstanding Fellow Teaching Award, 2015 - 2016
• Pediatric Infectious Diseases Society Fellow Video Award, 2015
• Publons Peer Review Award, Top 1% in Microbiology, 2018
• St. Jude/PIDS Pediatric Infectious Diseases Research Conference Poster Award, 2019
• American Association of Immunology Early Career Travel Award, 2020

Recent Publications view all (12)

Publication Co-Authors

Gregory A. Storch, M.D.

David A. Hunstad, M.D.

Kristine Wylie, Ph.D.

Todd N. Wylie

S. Celeste Morley, M.D., Ph.D.

Washington University has a long tradition of collaboration amongst colleagues.The faculty above have co-authored publications with David A. Rosen, M.D., Ph.D.

1. Twentyman J, Morffy Smith C, Nims JS, Dahler AA, Rosen DA. A murine model demonstrates capsule-independent adaptive immune protection in survivors of Klebsiella pneumoniae respiratory tract infection. Dis Model Mech. 2020;13(3). PMCID:PMC7104859  PMID:32298236 
2. Feldman MF, Mayer Bridwell AE, Scott NE, Vinogradov E, McKee SR, Chavez SM, Twentyman J, Stallings CL, Rosen DA, Harding CM. A promising bioconjugate vaccine against hypervirulent Klebsiella pneumoniae. Proc Natl Acad Sci U S A. 2019;116(37):18655-18663. PMCID:PMC6744904  PMID:31455739 
3. Schwartz DJ, Elward A, Storch GA, Rosen DA. Ureaplasma urealyticum pyelonephritis presenting with progressive dysuria, renal failure, and neurologic symptoms in an immunocompromised patient. Transpl Infect Dis. 2019;21(2):e13032. PMCID:PMC6542459  PMID:30472777 
4. Wylie KM, Wylie TN, Minx PJ, Rosen DA. Whole-genome sequencing of Klebsiella pneumoniae isolates to track strain progression in a single patient with recurrent urinary tract infection. Front Cell Infect Microbiol. 2019;9:14. PMCID:PMC6375827  PMID:30800643 
5. Potter RF, Lainhart W, Twentyman J, Wallace MA, Wang B, Burnham CA, Rosen DA, Dantas G. Population Structure, Antibiotic Resistance, and Uropathogenicity of Klebsiella variicola. MBio. 2018;9(6). PMCID:PMC6299229  PMID:30563902 
6. Svetlov D, Shi D, Twentyman J, Nedialkov Y, Rosen DA, Abagyan R, Artsimovitch I. In silico discovery of small molecules that inhibit RfaH recruitment to RNA polymerase. Mol Microbiol. 2018;110(1):128-142. PMCID:PMC6595482  PMID:30069925 
7. Rosen DA, Twentyman J, Hunstad DA. High levels of cyclic di-GMP in Klebsiella pneumoniae attenuate virulence in the lung. Infect Immun. 2018;86(2). PMCID:PMC5778367  PMID:29158434 
8. Rosen DA, Hilliard JK, Tiemann KM, Todd EM, Morley SC, Hunstad DA. Klebsiella pneumoniae FimK promotes virulence in murine pneumonia. J Infect Dis. 2016;213(4):649-58. PMCID:PMC4721909  PMID:26347570 
9. Rosen DA, Hung CS, Kline KA, Hultgren SJ. Streptozocin-induced diabetic mouse model of urinary tract infection. Infect Immun. 2008;76(9):4290-8. PMCID:PMC2519435  PMID:18644886 
10. Rosen DA, Pinkner JS, Jones JM, Walker JN, Clegg S, Hultgren SJ. Utilization of an intracellular bacterial community pathway in Klebsiella pneumoniae urinary tract infection and the effects of FimK on type 1 pilus expression. Infect Immun. 2008;76(7):3337-45. PMCID:PMC2446714  PMID:18411285 
11. Rosen DA, Pinkner JS, Walker JN, Elam JS, Jones JM, Hultgren SJ. Molecular variations in Klebsiella pneumoniae and Escherichia coli FimH affect function and pathogenesis in the urinary tract. Infect Immun. 2008;76(7):3346-56. PMCID:PMC2446687  PMID:18474655 
12. Rosen DA, Hooton TM, Stamm WE, Humphrey PA, Hultgren SJ. Detection of intracellular bacterial communities in human urinary tract infection. PLoS Med. 2007;4(12):e329. PMCID:PMC2140087  PMID:18092884